DNA

Part:BBa_K3476003

Designed by: Daria Shumkova   Group: iGEM20_MSP-Maastricht   (2020-10-17)


shRNA Pro2_c (Thaumetopoea processionea)

Part is encoding a short hairpin RNA against the Pro2-gene in Oak Processionary Catterpillars (Thaumetopoea processionea). Made for restriction cloning with EcoR1 & BamH1 into vector L4440.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 20
    Illegal BamHI site found at 37
    Illegal BamHI site found at 54
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part
//function/degradation
Parameters
None